| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Collazo, Isaiah J (9)
|
PVFISH |
44.03 |
35.64 |
-8.39 |
| 2 |
Yu, Felix P (10)
|
PVMACH |
44.76 |
35.99 |
-8.77 |
| 3 |
Bell, Zachary D (10)
|
PVNCAP |
36.56 |
36.24 |
-0.32 |
| 4 |
Chung, Eugene (10)
|
PVSSCT |
46.18 |
37.38 |
-8.80 |
| 5 |
Fulham, Weston J (10)
|
PVNCAP |
41.66 |
39.43 |
-2.23 |
| 6 |
Kownacki, Cooper F (9)
|
PVMACH |
42.29 |
41.73 |
-0.56 |
| 7 |
Testani, Joseph D (10)
|
PVNCAP |
|
41.79 |
- |
| 8 |
Li, Leo K (9)
|
PVNCAP |
44.26 |
43.06 |
-1.20 |
| 9 |
Tajima, Hideaki (10)
|
PVFISH |
56.00 |
43.79 |
-12.21 |
| 10 |
Jones, Luke P (10)
|
PVMACH |
43.47 |
44.82 |
1.35 |
| 11 |
Mirali, Sina N (10)
|
PVFISH |
50.18 |
45.25 |
-4.93 |
| 12 |
Jemio, Gabriel M (10)
|
PVFISH |
|
45.90 |
- |
| 13 |
Phillips, Chase J (10)
|
PVNCAP |
47.94 |
46.15 |
-1.79 |
| 14 |
Koay, Ethan P (10)
|
PVFISH |
54.08 |
46.55 |
-7.53 |
| 15 |
Nagle, Ryan P (10)
|
PVMACH |
49.38 |
47.46 |
-1.92 |
| 16 |
Hwang, Zeno (10)
|
PVSSCT |
|
48.67 |
- |
| 17 |
Teufel, Pierce A (9)
|
PVMACH |
54.47 |
49.43 |
-5.04 |
| 18 |
Southworth, Andrew B (9)
|
PVFISH |
|
49.99 |
- |
| 19 |
Yeh, Reid F (9)
|
PVMACH |
|
50.18 |
- |
| 20 |
Kim, Christian Y (9)
|
PVFISH |
|
50.66 |
- |
| 21 |
Bockman, Elijah L (9)
|
PVFISH |
1:00.47 |
50.73 |
-9.74 |
| 22 |
Osten, Elijah E (10)
|
PVMACH |
52.55 |
53.93 |
1.38 |
| 23 |
Li, William Y (10)
|
PVFISH |
|
54.57 |
- |
| 24 |
Kownacki, Ryan E (10)
|
PVMACH |
|
55.16 |
- |
| 25 |
Rieger, Jacob D (10)
|
PVNCAP |
|
56.46 |
- |
| 26 |
Zhao, Darren D (9)
|
PVFISH |
|
57.08 |
- |
| 27 |
Park, Dylan J (9)
|
PVMAC |
|
57.30 |
- |
| 28 |
Bradford, Benjamin J (9)
|
PVMACH |
1:04.28 |
58.57 |
-5.71 |
| 29 |
Xing, Aspen N (9)
|
PVFISH |
|
1:01.13 |
- |
| 30 |
Eslinger, Juan M (9)
|
PVFISH |
|
1:10.70 |
- |
| 31 |
Isaza, Matthew A (10)
|
PVMACH |
|
1:18.68 |
- |
|
Kim, Luke M (10)
|
PVMAC |
|
DQ |
- |
|
Pizzamiglio, Alexander J (9)
|
PVFISH |
|
DQ |
- |
|
Kim, Connor D (10)
|
PVMAC |
51.77 |
DQ |
- |
|
Yun, Colin (10)
|
PVSSCT |
|
NS |
- |